Journal: Biomedicines

Article Title: Acetylated Diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol in Autoimmune Arthritis and Interstitial Lung Disease in SKG Mice

doi: 10.3390/biomedicines9091095

Figure Lengend Snippet: Histologic examination of the lung tissue from SKG mice at 20 weeks after a curdlan injection with/without PLAG treatment. ( A ) Hematoxylin and eosin (upper panels) and Masson’s trichrome (lower panels) staining of the lung tissues. Severe lung inflammations were observed in the curdlan-induced SKG mice and these features were attenuated in the PLAG-treated curdlan-induced SKG mice. ( B ) Opal multiplexed immunofluorescent images and semi-quantitation of the total, GM-CSF + and IL-17A + neutrophil accumulation in the lung tissues. The GM-CSF + neutrophil accumulation was significantly decreased in the PLAG-treated curdlan-induced SKG mice compared with those without PLAG treatment whereas the IL-17A + neutrophil accumulation was not attenuated. The values represent the mean of three independent experiments ± SEM. * p < 0.05. PLAG: acetylated diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol.

Article Snippet: The slides were again placed in a citrate buffer (pH 6.0), heated in a microwave and incubated with primary rabbit antibodies for IL-17A (Novus Biologicals, Centennial, CO, USA, NBP1-76337, 1:500) for 1 h in a humidified chamber at RT followed by a detection using polymer HRP Ms + Rb.

Techniques: Injection, Staining, Quantitation Assay

Journal: Experimental neurology

Article Title: Critical role of EphA4 in early brain injury after subarachnoid hemorrhage in rat

doi: 10.1016/j.expneurol.2017.07.003

Figure Lengend Snippet: Effect of Ephrin receptor A4 (EphA4) knockdown on apoptotic neuron death after SAH. (A) Representative images of Fluoro-Jade staining at 24 h hours after SAH. (B) Quantitative analyses of mean apoptotic cell counts from 8 fields of left hemisphere at 24 h hours after SAH (NO/mm2). n = 3 per group. Vehicle, phosphate-buffered saline; siRNA, small interfering ribonucleic acid; rEphx-1, recombinant Ephexin-1. Top panel indicates the location of staining (small black box). *P < 0.05 vs. Sham; @P < 0.05 vs. Vehicle; #P < 0.05 vs. Scramble siRNA; &P < 0.05 vs. EphA4 siRNA; $P < 0.05 vs. EphA4 siRNA + rEphx-1. Bar = 50 μm.

Article Snippet: Fluoro-Jade C staining Apoptotic neuron death was observed by Fluoro-Jade C (TR-100-FJ, Biosensis, USA) staining, which was performed at 24 h after SAH as the instruction of the kit.

Techniques: Knockdown, Staining, Saline, Recombinant

Journal: bioRxiv

Article Title: Integrin α6β4 signaling switches DNA repair from homologous recombination to non-homologous end-joining pathway to sensitize breast cancer cells to cisplatin

doi: 10.1101/785873

Figure Lengend Snippet: BT549 cells (EV and β4) plated on laminin-1 and treated with 10 μM cisplatin for 24 hrs were harvested for cytosolic or nuclear fractions and immuno-blotted for phospho-p53 using actin and p84 as controls (A) or for immunocytochemistry staining for phospho-p53 S15 and phospho-53BP1 S1778 as indicated (B, scale bars, 20µm). (C) Subcellular protein fractionation was performed on cells treated as in (A) and noted fractions were immunoblotted with DDR proteins as indicated. Tubulin was used as the marker for total and cytosolic protein, Histone H2B and p84 were used as the markers for nuclear fractions.

Article Snippet: Primary antibodies used were mouse or rabbit anti-p53 (1:100, Cell Signaling), mouse anti-DNA-PKc (1:100, Cell Signaling), and rabbit anti-53BP1 (1:100, cell signaling).

Techniques: Immunocytochemistry, Staining, Fractionation, Marker

Journal: bioRxiv

Article Title: Integrin α6β4 signaling switches DNA repair from homologous recombination to non-homologous end-joining pathway to sensitize breast cancer cells to cisplatin

doi: 10.1101/785873

Figure Lengend Snippet: BT549 cells (EV and β4) were plated on laminin-1-coated plates, pretreated with DNA-PKc inhibitors NU7441(A) or NU7026 (B) at indicated concentrations for 1 hr before treatment with 10 µM cisplatin for 24hrs. Cell lysates were then immunoblotted for signaling proteins in DNA repair pathway as noted. (C) BT549 cells (EV and β4) plated on laminin-1-coated coverslips were treated with 10 µM cisplatin for 24hrs, the associations of DNA-PKc, p53, and 53BP1 were assessed by PLA, as noted. Scale bars, 10µm.

Article Snippet: Primary antibodies used were mouse or rabbit anti-p53 (1:100, Cell Signaling), mouse anti-DNA-PKc (1:100, Cell Signaling), and rabbit anti-53BP1 (1:100, cell signaling).

Techniques: